(Natural News) In today’s Situation Update podcast (below), I share passages from the Old Testament that are highly relevant today when it comes to protecting children against being sacrificed to Satan.
In Leviticus, God explains (through Moses) that not only is child sacrifice sinful and evil, but that communities have an obligation to stop parents who are sacrificing their children to Moloch (“Molek”).
Societies that fail to stop the practice of child sacrifice face the wrath of God. Here’s Leviticus 20:2-5 (emphasis added)
Say to the Israelites: ‘Any Israelite or any foreigner residing in Israel who sacrifices any of his children to Molek is to be put to death. The members of the community are to stone him. I myself will set my face against him and will cut him off from his people; for by sacrificing his children to Molek, he has defiled my sanctuary and profaned my holy name. If the members of the community close their eyes when that man sacrifices one of his children to Molek and if they fail to put him to death, I myself will set my face against him and his family and will cut them off from their people together with all who follow him in prostituting themselves to Molek.’
As you can see, God calls for harsh punishment for parents who sacrifice their own children. In Exodus, God also shows that he is willing to unleash plagues upon entire nations that fail to follow his teachings. His plagues become increasingly severe as his warnings are ignored. In Exodus, God commanded the Pharaoh to set free the Israelites, and the Pharaoh refused until the tenth plague finally changed his mind.
Notably, at least two of these plagues are biological weapons attacks (livestock disease and the plague of the boils). Other plagues unleashed by God against Egypt include locusts, flies, gnats, frogs and finally, the mass death of all first-born sons.
Spike protein vaccines are biological weapons aimed at human children
Covid vaccines contain instructions for the body to manufacture spike protein nanoparticles. Those toxic nanoparticles attack the body from the inside and even damage the DNA repair mechanism found in the nuclei of human cells. Accordingly, covid vaccines are a biological weapon being used to destroy human lives.
When they are injected into children with the permission of their parents, those parents are sacrificing their children and condemning them to death. God has already chimed in on his disdain for those parents who sacrifice their own children, and if the modern world does not stop this medical violence being committed against children, it’s quite clear that God will unleash his wrath against the nations of our world.
✴️ Stop what you’re doing and see this now! MILLIONS are protesting in non-stop demonstrations all across Italy! They’re not only fighting for their own children, they’re fighting to MAKE SURE these heinous laws do not pass in other countries. UNITY is an unstoppable force! Still think vaccines are safe? Check this out while it’s still playing! tinyurl.com/9Episodes <<< Revisions to the Italian mandate have already been made thanks to these protests, yet Italy will NOT QUIT until there are no tyrannical mandates at ALL!
✴️ Networking, exemption information and doctor resources: tinyurl.com/RevolutionForVaccineChoice
✴️ Follow us: facebook.com/RevolutionForChoice
✴️ Read all vaccine inserts: tinyurl.com/ReadTheVaccineInsert #RevolutionForChoice #InformedConsent #EducateBeforeYouVaccinate #VAXXED #Italy #Unity #NonCompliance #SB277 #Freedom
Co-Author of Lancet MMR-Autism Study Exonerated on All Charges of Professional Misconduct
Dr. John Walker-Smith, considered the father of pediatric gastroenterology, has today been restored to his much-deserved reputation of high esteem. His appeal of the U.K. General Medical Council’s 2010 decision to remove his license for serious professional misconduct has been quashed in its entirety. This decision raises questions about the validity of the 2010 GMC proceeding in general.
The GMC proceeding was a multi-year, multi-million dollar prosecution against Drs. Wakefield, Walker-Smith, and Murch. It related to a controversial 1998 study published in The Lancet suggesting a possible link between autism, the measles-mumps-rubella vaccine and bowel disease. Based on the GMC prosecution, both Drs. Walker-Smith and Wakefield lost their licenses to practice and the Lancet article was officially retracted. The GMC alleged that the physician-authors had failed to obtain necessary ethical clearances and that they had subjected the twelve children in the study to unnecessary medical procedures.
Justice Mitting, reviewing Dr. Walker-Smith’s appeal in the High Court of Justice, Queen’s Bench Division, Administrative Court, found that the GMC’s conclusions were “based on inadequate and superficial reasoning” and that “the finding of serious professional misconduct and the sanction of erasure are both quashed.” See full text of the decision
Repeal Immunity for Drug Companies Against Vaccine Injuries
Why should the drug companies be above the law? If vaccines are safe, there would be no need to grant the drug companies immunity. In 1986 Congress gave the drug companies immunity against all lawsuits from vaccine related injuries. The Federal Government is now paying out billions in damages to some parents whose children have been hurt by vaccines. While the drug companies continue to rack up huge profits, most families continue to pay for the damages with their own money.
Robert F. Kennedy, Jr.: Doing the math on meningitis vaccinations
By Robert F. Kennedy, Jr.
Posted: 06/09/2015 07:55:00 PM MDT
This past Thursday, the University of Colorado-Boulder student government passed a resolution asking the CDC’s Advisory Committee on Immunization Practices (ACIP) to recommend meningococcal vaccines for all incoming college students. ACIP will consider CU-Boulder’s resolution on June 24. ACIP’s first agenda topic will be the debate over recommending the meningococcal B vaccines for adolescents and college students.
With billions of dollars in annual revenue at stake, vaccine makers are pushing meningitis vaccine mandates across the country. Vaccine issues are always complex, but advocates of the meningitis mandate should consider some simple math.
Meningococcal meningitis is exceedingly rare. There were only about 390 cases in the U.S. last year. In a population of 319 million, that adds up to one case in 817,949 people. There were only three meningococcal meningitis cases in Colorado last year — one resulting in death. The CDC has approved three vaccines targeting the A, C, Y and W135 strains of meningitis: Menactra, Menveo and Menomune, which still contains significant mercury concentrations in multi-dose vials. These vaccines are effective in providing immunity to those strains of meningitis in only 85 percent of people who receive them.
Thirty percent of the meningitis cases are the B strain, which typically occur in college-aged kids and against which the three vaccines are completely ineffective.
The FDA recently approved two B strain meningococcal vaccines, Trumenba and Bexsero.Vaccine makers are pushing government officials to add them to the recommended schedule for the fall semester. Critics have faulted the government’s expedited safety and efficiency testing for the new B strain vaccines citing glaring lapses in safety protocols including the absence of inactive placebos. In addition, both new B vaccines are “pregnancy category B,” meaning that they should be administered to pregnant women only when necessary. Neither vaccine has been tested for carcinogenicity, mutagenicity or effects on male fertility.
According to their package inserts, Menactra and Menveo produce “serious adverse events” in 1 percent of recipients. Menomune, with its hefty mercury load, sickens 1.3 percent of those receiving it. According to the CDC Pink Book, 0.3 percent of those with “serious adverse events” from meningitis vaccines will die. So here is the math calculation that thoughtful student governments in Colorado must consider: If you inoculate Colorado’s 400,000 college students with the older vaccines, you can expect 4,000 serious adverse events and 12 dead. We do not yet know the effects of widespread vaccination of the hastily-expedited B vaccines, but according to their package inserts, about 2 percent of students who receive the B vaccine will be sickened or hospitalized with a serious adverse event. This could translate into an additional 8,000 sick students and 24 deaths, for a total of 12,000 sick and 36 dead in the attempt to possibly avert three meningitis cases.
Sanjay Gupta MD has to admit ON CNN that a Neurologist’s (yes a doctor’s) daughter’s autism was caused by vaccines.
I think it pains him… He says this is “very rare” in the video. Thoughts?
Story at-a-glance
Groundbreaking new study identifies vaccines as the most common source of adverse drug reactions in children, including anaphylaxis and death
Study reveals adverse drug reactions are more common for boys than girls, and infants are the most susceptible age group
Vaccines provide only artificial, temporary, typically inferior immunity compared to what your body receives from natural exposure to a disease
History offers little evidence that vaccines are responsible for eradicating disease even when “herd immunity” vaccination levels have been reached
After vaccines are introduced, new and more virulent strains tend to emerge, suggesting the entire vaccine-immunity paradigm is fatally flawed
By Dr. Mercola
A groundbreaking new drug safety study in Shanghai, China, provides some much needed information about the frequency of vaccine drug reactions among children.
Adverse drug reactions are a serious public health concern and one of the leading causes of morbidity and mortality worldwide.1 More than a half million children are treated every year for adverse drug reactions in US outpatient clinics and emergency rooms.2
The Shanghai study, based on reported pediatric adverse drug reactions (ADRs) for 2009, found that 42 percent were caused by vaccines, with reactions ranging from mild skin rashes to deadly reactions like anaphylaxis and death. Of all the drugs causing adverse reactions among children, vaccines are the most commonly reported.3, 4
This study is particularly significant because the vast majority of reports came from physicians, pharmacists, and other health care providers. Less than three percent of the reports were from consumers.
Another safety report5 about pediatric drug reactions was just published by the Institute for Safe Medication Practices (ISMP) and lists the top 15 drugs causing serious adverse reactions in children.
Psychiatric drugs and analgesics (especially ibuprofen) figure prominently in their top 15 list. The report also mentions psychological side effects such as aggression and suicidal ideation as frequent symptoms from 10 of the 15 most commonly reported drugs.
The Price of Prevention: Vaccine Costs Are Soaring
By ELISABETH ROSENTHALJULY 2, 2014
SAN ANTONIO — There is little that Dr. Lindsay Irvin has not done for the children’s vaccines in her office refrigerator: She remortgaged her home to afford their rising prices. She packed them in ice chests and moved them when her office flooded this year. She pays a company to monitor the fridge in case the temperature rises.
“The security company can call me any time of the day or night so I can go save my vaccines,” said Dr. Irvin, a pediatrician. Those in the refrigerator recently cost $70,000, she said — “more than I paid for four years of medical school.”
Vaccination prices have gone from single digits to sometimes triple digits in the last two decades, creating dilemmas for doctors and their patients as well as straining public health budgets. Here in San Antonio and elsewhere, some doctors have stopped offering immunizations because they say they cannot afford to buy these potentially lifesaving preventive treatments that insurers often reimburse poorly, sometimes even at a loss.
Childhood immunizations are so vital to public health that the Affordable Care Act mandates their coverage at no out-of-pocket cost and they are generally required for school entry. Once a loss leader for manufacturers, because they are often more expensive to produce than conventional drugs, vaccines now can be very profitable.
Old vaccines have been reformulated with higher costs. New ones have entered the market at once-unthinkable prices. Together, since 1986, they have pushed up the average cost to fully vaccinate a child with private insurance to the age of 18 to $2,192 from $100, according to data from the Centers for Disease Control and Prevention. Even with deep discounts, the costs for the federal government, which buys half of all vaccines for the nation’s children, have increased 15-fold during that period. The most expensive shot for young children in Dr. Irvin’s refrigerator is Prevnar 13, which prevents diseases caused by pneumococcal bacteria, from ear infections to pneumonia.
Vaccines may trigger autoimmune diseases
Apr 12, 2015
Anti-vaxxers were severely criticized following the Disneyland measles outbreak, but research shows their reasons for not wanting to vaccinate their children might be well-founded.
Nowadays, newborns are injected with as many as over 30 vaccines before the age of three, but the array of non-ingredients that are found in vaccines have been shown to be unsafe. Yehuda Shoenfeld, also known as the “Godfather of Autoimmunology” is an Israeli clinician who has spent over three decades studying the human immune system. According to a recent article published in the journal Pharmacological Research, the physician joined forces with some of his colleagues and concluded that vaccines [their ingredients, specifically aluminium] represent a significant contributor to the soaring global epidemic of autoimmune diseases. In the article, Dr Shoenfeld and his colleagues issued unprecedented guidelines identifying four categories of people who are most at risk for autoimmunity induced by vaccine.
An autoimmune disorder occurs when the immune system wrongly attacks and destroys healthy body tissue. There are currently over 80 different types of autoimmune disorders. The paper’s authors acknowledge that vaccines prevent infections that can cause autoimmunity, but they also accept conclusions issued by plenty of reports, namely that vaccines can also trigger autoimmunity. Autoimmune diseases which can occur post-vaccination are lupus, arthritis, diabetes mellitus and others, but “almost all types of vaccines have been reported to be associated with the onset of ASIA.”
ASIA is “a wide concept that includes any environmental factor which is demonstrated to trigger autoimmune conditions,” according to Ignasi Rodriguez-Pinto, autoimmunologist at the Barcelona Hospital Clinic. The umbrella term was first identified by Dr Shoenfeld in the Journal of Autoimmunology in 2011 and includes a large spectrum of neurological and immune-mediated phenomena observed following vaccine injections that result from exposure to ingredients such as aluminium. Dr Rodriguez-Pinto pointed out that vaccination for Hepatitis B can also trigger ASIA. The “Grandfather of Autoimmunology” wrote in the paper published in the journal Pharmacological Research that “throughout our lifetime, the normal immune system walks a fine line between preserving normal immune reactions and developing autoimmune diseases.”
Five Year Moratorium on Childhood Vaccines
Created by K.H. on July 03, 2017
American children are in crisis with an explosion of once-rare neurological problems like autism and seizures.
Recent scientific evidence has shown massive contamination of vaccines with unsuspected chemical and biological agents. Other evidence shows dramatic differences in in health outcomes between vaccinated and un-vaccinated children. In order to remedy this we ask the White House to:
ONE: Impose a five year moratorium on all childhood vaccines from birth to age eighteen. TWO: Repeal the 1986 National Childhood vaccine injury Act and return vaccines to the traditional civil justice system. THREE: Perform large scale studies of vaccinated and un-vaccinated children. FOUR: Ban direct pharmaceutical advertising to consumers and allow such advertising only to medical professionals
Can a routine holiday jab send you mad? That’s what BBC reporter Malcolm Brabant says happened to him – and he’s not alone
BBC foreign correspondent Malcolm Brabant had a yellow fever vaccine
24 hours later he had been reduced to a sweating, shivering wreck
Had a fever for two weeks then psychosis: ‘I went completely bonkers’
Spent two years in and out of mental institutions, unable to work
Even attempted suicide, but has fortunately made a full recovery
By Anna Van Praagh For The Daily Mail
Published: 22:21, 6 April 2015 | Updated: 12:31, 7 April 2015
Zero Hedge – U.S. Journalist Investigating “Migrant Crime” In Sweden Has To Leave Under “Police Escort” For His Own Safety
After Trump called attention to the rising tide of migrant crime in Sweden last week, independent journalist Tim Pool decided to head to Stockholm for a first-hand look at the “crime-ridden migrant suburb” of Rinkeby. Unfortunately, upon arrival Pool didn’t get to do much investigating before arousing the suspicion of a couple of masked men who proceeded to follow him through the streets eventually leading to his prompt police escort out of the city out of fear for his crew’s safety.
Zero Hedge – Former Marine’s Facebook Post On VA Treatment Of Patients Causes Outrage
A former Marine and his wife say the scene inside a Veterans Administration hospital in Durham, North Carolina, was so shocking that they took pictures and posted them to Facebook.
Now, as AP reports, thousands of people are expressing outrage and the head of the medical center says an employee involved has been removed from patient care pending an internal review.
If You See THIS Label On the Fruit Do Not Buy It at Any Cost!
by ANYA V
Most of us don’t know that the stickers attached to the fruits and vegetables are there for more than just scanning the price.
The PLU code, or the price lookup number on the sticker can help you determine if the product is genetically modified, organic or produced with chemical fertilizers, fungicides or herbicides.
1. A four-digit code beginning with a 3 or a 4 means the produce is probably conventionally grown. The last digits of the code represent the kind of the fruit or vegetable you are buying. For example, bananas are always labeled with the code of 4011.
2. If there are five numbers, and the first is “8″, then the product is genetically modified. The label on genetically modified banana (GE-genetically engineered of GMO) would contain the numbers 84011.
3. A five-digit number that starts with a 9 means the item is organic. Organic bananas are labeled with 94011.
EWG ( The Environmental Working Group) analyzed pesticide residue testing data from the U.S. Department of Agriculture and Food and Drug Administration to come up with rankings for these popular fresh produce items.
ONE GERMAN WEEKEND: TWO CASES OF ‘SOUTHERN TYPES’ GANG-RAPING WOMEN
Posted on March 1, 2017 11:00
By David Frankenhuis
Two women in Germany last weekend became the victim of brutal sexual assaults in two separate incidents. In both cases, the attackers were described as ‘southern types’, operating in small bands.
Carnival rape, crying woman in Leverkusen
On Sunday night, a 31-year-old woman in the city of Leverkusen was victimised by a gang of three men in the district of Lützenkirchen. She was attacked by all three of the perpetrators and subsequently raped on a lawn by one of them. After the assault, the men fled in an unknown direction, Freie Zeiten reports.
Passers-by had, later on, found the crying woman sitting in the streets. She was dressed up as a hippie since she had been out celebrating Carnival.
According to the victim, the men were dressed in dark clothes, in between 20 and 30 years of age, and all had a ‘southern appearance’. This usually means they come from countries such as Algeria, Tunisia, Morocco and Egypt.
The police are asking the public for help, and an arrest has yet to be made in this case.
Violent gang rape on Hamburg riverbank
In Hamburg, two men on Sunday evening sexually assaulted a 46-year-old woman after beating her. The violent gang rape took place near a bridge called the Lombardsbrücke, which is located on the riverbank of the Alster.
On Monday morning, the injured victim went to a hospital where she was investigated and treated, Focus.de and other local media report. Afterwards, she spoke to police to file a complaint.
In this case, as well, the woman referred to the rapists as being of southern descent, meaning Northern Africans or, in German police lingo, the so-called ‘Nafri’s’. She could not describe the assailants more accurate.
During the interrogation, the victim mentioned an elderly couple passing by the location of the attack. They may be important witnesses, police have stated.
Cases of mumps hits 10-year high
COLORADO SPRINGS, Colo. (KXRM)— Top health officials are monitoring the worst mumps in the United States in 10 years.
Harvard University has been dealing with handfuls of infected students since the beginning of the school year and over at the University of Missouri, more than 200 people have come down with the virus.
The CDC says mumps cases have now surfaced in all but four states with nearly 4,300 infections reported.
Several public schools near Seattle have reported a growing number of cases.
Arkansas is the epicenter – with nearly 2,200 cases – in mostly school-aged children.
Mumps is extremely contagious and can be spread with a sneeze or cough.
Doctors say the best way to protect yourself is to get vaccinated.
Public Health Officials Know: Recently Vaccinated Individuals Spread Disease
Washington, D.C., March 3, 2015 (GLOBE NEWSWIRE) — Physicians and public health officials know that recently vaccinated individuals can spread disease and that contact with the immunocompromised can be especially dangerous. For example, the Johns Hopkins Patient Guide warns the immunocompromised to “Avoid contact with children who are recently vaccinated,” and to “Tell friends and family who are sick, or have recently had a live vaccine (such as chicken pox, measles, rubella, intranasal influenza, polio or smallpox) not to visit.”1
A statement on the website of St. Jude’s Hospital warns parents not to allow people to visit children undergoing cancer treatment if they have received oral polio or smallpox vaccines within four weeks, have received the nasal flu vaccine within one week, or have rashes after receiving the chickenpox vaccine or MMR (measles, mumps, rubella) vaccine.2
“The public health community is blaming unvaccinated children for the outbreak of measles at Disneyland, but the illnesses could just as easily have occurred due to contact with a recently vaccinated individual,” says Sally Fallon Morell, president of the Weston A. Price Foundation. The Foundation promotes a healthy diet, non-toxic lifestyle and freedom of medical choice for parents and their children. “Evidence indicates that recently vaccinated individuals should be quarantined in order to protect the public.”
Scientific evidence demonstrates that individuals vaccinated with live virus vaccines such as MMR (measles, mumps and rubella), rotavirus, chicken pox, shingles and influenza can shed the virus for many weeks or months afterwards and infect the vaccinated and unvaccinated alike.
Measles Transmitted By The Vaccinated, Gov. Researchers Confirm
A remarkable study reveals that a vaccinated individual not only can become infected with measles, but can spread it to others who are also vaccinated against it – doubly disproving two doses of MMR vaccine is “99% effective,” as widely claimed.
One of the fundamental errors in thinking about measles vaccine effectiveness is that receipt of measles-mumps-rubella (MMR) vaccine equates to bona fide immunity against these pathogens. Indeed, it is commonly claimed that receiving two doses of the MMR vaccine is “99 percent effective in preventing measles,”1 despite a voluminous body of contradictory evidence from epidemiology and clinical experience.
This erroneous thinking has led the public, media and government alike to attribute the origin of measles outbreaks, such as the one recently reported at Disney, to the non-vaccinated, even though 18% of the measles cases occurred in those who had been vaccinated against it — hardly the vaccine’s claimed “99% effective.” The vaccine’s obvious fallibility is also indicated by the fact that that the CDC now requires two doses.
But the problems surrounding the failing MMR vaccine go much deeper. First, they carry profound health risks (over 25 of which we have indexed here: MMR vaccine dangers), including increased autism risk, which a senior CDC scientist confessed his agency covered up. Second, not only does the MMR vaccine fail to consistently confer immunity, but those who have been “immunized” with two doses of MMR vaccine can still transmit the infection to others — a phenomena no one is reporting on in the rush to blame the non- or minimally-vaccinated for the outbreak.
MMR Vaccinated Can Still Spread Measles
Last year, a groundbreaking study published in the journal Clinical Infectious Diseases, whose authorship includes scientists working for the Bureau of Immunization, New York City Department of Health and Mental Hygiene, and the National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, GA, looked at evidence from the 2011 New York measles outbreak that individuals with prior evidence of measles vaccination and vaccine immunity were both capable of being infected with measles and infecting others with it (secondary transmission).
This finding even aroused the attention of mainstream news reporting, such as this Sciencemag.org article from April 2014 titled “Measles Outbreak Traced to Fully Vaccinated Patient for First Time.”
Titled, “Outbreak of Measles Among Persons With Prior Evidence of Immunity, New York City, 2011,” the groundbreaking study acknowledged that, “Measles may occur in vaccinated individuals, but secondary transmission from such individuals has not been documented.”
In order to find out if measles vaccine compliant individuals are capable of being infected and transmitting the infection to others, they evaluated suspected cases and contacts exposed during a 2011 measles outbreak in NYC. They focused on one patient who had received two doses of measles-containing vaccine and found that,
Rise In Mumps Cases Has Some Public Health Officials Asking Questions
Caroline Brown, a sophomore at the University of Missouri got a fever over Thanksgiving break. Soon it became painful to bite down, and her cheek began to swell. A trip to her physician confirmed it – Caroline had the mumps.
“Mumps kind of sounds like this archaic thing,” Brown said. “We get vaccinated for it – it just sounds like something that nobody gets. So I just didn’t think that it was possible that I would get it.”
But mumps is back, and is having its worst year in a decade, fueled in part by its spread on college campuses. Since classes began at the University of Missouri in August, school officials have identified 193 mumps cases on campus, with more unreported cases likely. And nationwide more than 4,000 cases have been reported to the CDC, nearly triple the cases in 2015 and the largest spike in 10 years.
The spike has some public health officials asking questions about the existing vaccine protocol.
Dr. Susan Even, executive director for the University of Missouri’s Student Health Center said she hasn’t seen anything like the current outbreak in her 31 years at the school. She said all of the students her team treated for mumps had two MMR (Measles, Mumps and Rubella) vaccine doses — a school requirement — but they got sick anyway.
“The fact that we have mumps showing up in highly immunized populations likely reflects something about the effectiveness of the vaccine,” Even said.
Study – Real-time RT-PCR assays to differentiate wild-type group A rotavirus strains from Rotarix® and RotaTeq® vaccine strains in stool samples
Abstract
Group A rotaviruses (RVA) are the leading cause of severe diarrhea in young children worldwide. Two live-attenuated RVA vaccines, Rotarix® and RotaTeq® are recommended by World Health Organization (WHO) for routine immunization of all infants. Rotarix® and RotaTeq® vaccines have substantially reduced RVA associated mortality but occasionally have been associated with acute gastroenteritis (AGE) cases identified in vaccinees and their contacts. High-throughput assays are needed to monitor the prevalence of vaccine strains in AGE cases and emergence of new vaccine-derived strains following RVA vaccine introduction. In this study, we have developed quantitative real-time RT-PCR (qRT-PCR) assays for detection of Rotarix® and RotaTeq® vaccine components in stool samples. Real-time RT-PCR assays were designed for vaccine specific targets in the genomes of Rotarix® (NSP2, VP4) and RotaTeq® (VP6, VP3-WC3, VP3-human) and validated on sequence confirmed stool samples containing vaccine strains, wild-type RVA strains, and RVA-negative stools. For quantification, standard curves were generated using dsRNA transcripts derived from RVA gene segments. Rotarix® NSP2 and VP4 qRT-PCR assays exhibited 92–100% sensitivity, 99–100% specificity, 94–105% efficiency, and a limit of detection of 2–3 copies per reaction. RotaTeq® VP6, VP3-WC3, and VP3-human qRT-PCR assays displayed 100% sensitivity, 94–100% specificity, 91–102% efficiency and limits of detection of 1 copy, 2 copies, and 140 copies, respectively. These assays permit rapid identification of Rotarix® and RotaTeq® vaccine components in stool samples from clinical and surveillance studies and will be helpful in determining the frequency of vaccine strain-associated AGE.
Real-time RT-PCR assays to differentiate wild-type group A rotavirus strains from Rotarix® and RotaTeq® vaccine strains in stool samples
Abstract
Group A rotaviruses (RVA) are the leading cause of severe diarrhea in young children worldwide. Two live-attenuated RVA vaccines, Rotarix® and RotaTeq® are recommended by World Health Organization (WHO) for routine immunization of all infants. Rotarix® and RotaTeq® vaccines have substantially reduced RVA associated mortality but occasionally have been associated with acute gastroenteritis (AGE) cases identified in vaccinees and their contacts. High-throughput assays are needed to monitor the prevalence of vaccine strains in AGE cases and emergence of new vaccine-derived strains following RVA vaccine introduction. In this study, we have developed quantitative real-time RT-PCR (qRT-PCR) assays for detection of Rotarix® and RotaTeq® vaccine components in stool samples. Real-time RT-PCR assays were designed for vaccine specific targets in the genomes of Rotarix® (NSP2, VP4) and RotaTeq® (VP6, VP3-WC3, VP3-human) and validated on sequence confirmed stool samples containing vaccine strains, wild-type RVA strains, and RVA-negative stools. For quantification, standard curves were generated using dsRNA transcripts derived from RVA gene segments. Rotarix® NSP2 and VP4 qRT-PCR assays exhibited 92–100% sensitivity, 99–100% specificity, 94–105% efficiency, and a limit of detection of 2–3 copies per reaction. RotaTeq® VP6, VP3-WC3, and VP3-human qRT-PCR assays displayed 100% sensitivity, 94–100% specificity, 91–102% efficiency and limits of detection of 1 copy, 2 copies, and 140 copies, respectively. These assays permit rapid identification of Rotarix® and RotaTeq® vaccine components in stool samples from clinical and surveillance studies and will be helpful in determining the frequency of vaccine strain-associated AGE.
Public Health Officials Know: Recently Vaccinated Individuals Spread Disease
Washington, D.C., March 3, 2015 (GLOBE NEWSWIRE) — Physicians and public health officials know that recently vaccinated individuals can spread disease and that contact with the immunocompromised can be especially dangerous. For example, the Johns Hopkins Patient Guide warns the immunocompromised to “Avoid contact with children who are recently vaccinated,” and to “Tell friends and family who are sick, or have recently had a live vaccine (such as chicken pox, measles, rubella, intranasal influenza, polio or smallpox) not to visit.”1
A statement on the website of St. Jude’s Hospital warns parents not to allow people to visit children undergoing cancer treatment if they have received oral polio or smallpox vaccines within four weeks, have received the nasal flu vaccine within one week, or have rashes after receiving the chickenpox vaccine or MMR (measles, mumps, rubella) vaccine.2
“The public health community is blaming unvaccinated children for the outbreak of measles at Disneyland, but the illnesses could just as easily have occurred due to contact with a recently vaccinated individual,” says Sally Fallon Morell, president of the Weston A. Price Foundation. The Foundation promotes a healthy diet, non-toxic lifestyle and freedom of medical choice for parents and their children. “Evidence indicates that recently vaccinated individuals should be quarantined in order to protect the public.”
Scientific evidence demonstrates that individuals vaccinated with live virus vaccines such as MMR (measles, mumps and rubella), rotavirus, chicken pox, shingles and influenza can shed the virus for many weeks or months afterwards and infect the vaccinated and unvaccinated alike.
the news network 