wild-type

Vaccine News – VAXXED TV – Something is Wrong & Study – Differentiation of RotaTeq® vaccine strains from wild-type strains using NSP3 gene in reverse transcription polymerase chain reaction assay

The JAMA Network – April 26, 2006

Study – Detection of Vaccinia DNA in the Blood Following Smallpox Vaccination

Routine administration of the smallpox vaccine ended in the United States in 1972. With the reinitiation of the US smallpox vaccination program in 2002, the risk of transmission of vaccinia virus from a recently vaccinated person to a susceptible host is a concern. Secondary transmission is biologically plausible because of evidence of viral persistence in vaccinees. Vaccinia virus has been cultured from the oropharynx of vaccine recipients with a normal course following vaccination.1 In the 1960s and 1970s, it was isolated from the blood and urine of a limited number of vaccine recipients who had complications following vaccination.2 More sensitive molecular techniques are now available for detecting viruses in clinical specimens. We describe findings using real-time polymerase chain reaction (PCR) to detect vaccinia DNA in smallpox vaccine recipients

US National Library of Medicine
National Institutes of Health – 2014

Study – Development, production, and postmarketing surveillance of hepatitis A vaccines in China.

Cui F, Liang X, Wang F, Zheng H, Hutin YJ, Yang W.
Author information
Chinese Center for Disease Control and Prevention.

Abstract
China has long experience using live attenuated and inactivated vaccines against hepatitis A virus (HAV) infection. We summarize this experience and provide recent data on adverse events after immunization (AEFIs) with hepatitis A vaccines in China. We reviewed the published literature (in Chinese and English) and the published Chinese regulatory documents on hepatitis A vaccine development, production, and postmarketing surveillance of AEFI. We described the safety, immunogenicity, and efficacy of hepatitis A vaccines and horizontal transmission of live HAV vaccine in China. In clinical trials, live HAV vaccine was associated with fever (0.4%-5% of vaccinees), rash (0%-1.1%), and elevated alanine aminotransferase (0.015%). Inactivated HAV vaccine was associated with fever (1%-8%), but no serious AEFIs were reported. Live HAV vaccine had seroconversion rates of 83% to 91%, while inactivated HAV vaccine had seroconversion rates of 95% to 100%. Community trials showed efficacy rates of 90% to 95% for live HAV and 95% to 100% for inactivated HAV vaccine. Postmarketing surveillance showed that HAV vaccination resulted in an AEFI incidence rate of 34 per million vaccinees, which accounted for 0.7% of adverse events reported to the China AEFI monitoring system. There was no difference in AEFI rates between live and inactivated HAV vaccines. Live and inactivated HAV vaccines manufactured in China were immunogenic, effective, and safe. Live HAV vaccine had substantial horizontal transmission due to vaccine virus shedding; thus, further monitoring of the safety of virus shedding is warranted.

US National Library of Medicine
National Institutes of Health – Nov 2016

Study – Differentiation of RotaTeq® vaccine strains from wild-type strains using NSP3 gene in reverse transcription polymerase chain reaction assay.

Jeong S – 1, Than VT – 1, Lim I – 2, Kim W – 3
Author information
1 Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
2 Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, South Korea.
3 Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea. Electronic address: kimwy@cau.ac.kr.

Abstract
RotaTeq® is a live attenuated human-bovine reassortant vaccine against rotaviruses that is used worldwide. However, shedding of the virus used in RotaTeq® has been detected in the feces of children following vaccination by the oral route, possibly affecting community immunity. Therefore, a simple and efficient method to discriminate between virulent and RotaTeq® vaccine strains is required. In this study, a novel one-step multiplex reverse-transcription polymerase chain reaction (RT-PCR) assay targeting the NSP3 gene was developed to detect RotaTeq® vaccine strains in fecal samples. RotaTeq® vaccine viruses were successfully distinguished from known wild-type rotavirus genotypes. In addition, the developed assay was able to detect rotaviruses in clinical stool samples obtained from South Korea during the 2011-2013 rotavirus seasons. Of the 1106 stool specimens from children with acute gastroenteritis that were screened, 286 rotaviruses were genotyped. RotaTeq® vaccine strains were identified in 39 samples (13.6%). The novel RT-PCR assay that was developed could be used to detect and discriminate between RotaTeq® vaccine strains that are shed in fecal matter, and to estimate the quantification of virus that has been shed after vaccination.

VAXXED TV – Something is Wrong

I Really Trusted Her

100% Proof! Human DNA in Vaccines

More M.D.s Come Forward

Adam’s Letter Board

We signed his life away

What kills 7,300 babies in the United States each year?

Do The Right Thing

Parents of Preteens Beware

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ONE FOR ISRAEL Ministry – Jewish Johnathan Ben-David forgave his killer and you would not believe why!!!

How to accept Jesus Christ as your personal Saviour

Testimony by Phil Robertson from Duck Dynasty

1 Corinthians 15 Authorized (King James) Version (AKJV)
1 Moreover, brethren, I declare unto you the gospel which I preached unto you, which also ye have received, and wherein ye stand;
2 by which also ye are saved, if ye keep in memory what I preached unto you, unless ye have believed in vain.
3 For I delivered unto you first of all that which I also received, how that Christ died for our sins according to the scriptures;
4 and that he was buried, and that he rose again the third day according to the scriptures:

Hebrews 6 Authorized (King James) Version (AKJV)
1 Therefore leaving the principles of the doctrine of Christ, let us go on unto perfection; not laying again the foundation of repentance from dead works, and of faith toward God,
2 of the doctrine of baptisms, and of laying on of hands, and of resurrection of the dead, and of eternal judgment.
3 And this will we do, if God permit.
4 For it is impossible for those who were once enlightened, and have tasted of the heavenly gift, and were made partakers of the Holy Ghost,
5 and have tasted the good word of God, and the powers of the world to come,
6 if they shall fall away, to renew them again unto repentance; seeing they crucify to themselves the Son of God afresh, and put him to an open shame.
7 For the earth which drinketh in the rain that cometh oft upon it, and bringeth forth herbs meet for them by whom it is dressed, receiveth blessing from God:
8 but that which beareth thorns and briers is rejected, and is nigh unto cursing; whose end is to be burned.

Isaiah 53 – Old testament Prophecy about Jesus

1 Who hath believed our report? and to whom is the arm of the Lord revealed?
2 For he shall grow up before him as a tender plant,and as a root out of a dry ground:he hath no form nor comeliness;and when we shall see him,there is no beauty that we should desire him.
3 He is despised and rejected of men;a man of sorrows, and acquainted with grief:and we hid as it were our faces from him;he was despised, and we esteemed him not.
4 Surely he hath borne our griefs,and carried our sorrows:yet we did esteem him stricken,smitten of God, and afflicted.
5 But he was wounded for our transgressions,he was bruised for our iniquities:the chastisement of our peace was upon him;and with his stripes we are healed.
6 All we like sheep have gone astray;we have turned every one to his own way;and the Lord hath laid on him the iniquity of us all.
7 He was oppressed, and he was afflicted,yet he opened not his mouth:he is brought as a lamb to the slaughter,and as a sheep before her shearers is dumb,so he openeth not his mouth.
8 He was taken from prison and from judgment:and who shall declare his generation? for he was cut off out of the land of the living:for the transgression of my people was he stricken.
9 And he made his grave with the wicked,and with the rich in his death;because he had done no violence,neither was any deceit in his mouth.
10 Yet it pleased the Lord to bruise him;he hath put him to grief:when thou shalt make his soul an offering for sin,he shall see his seed, he shall prolong his days,and the pleasure of the Lord shall prosper in his hand.
11 He shall see of the travail of his soul, and shall be satisfied:by his knowledge shall my righteous servant justify many;for he shall bear their iniquities.
12 Therefore will I divide him a portion with the great,and he shall divide the spoil with the strong;because he hath poured out his soul unto death:and he was numbered with the transgressors;and he bare the sin of many,and made intercession for the transgressors.

The Only Sin That Leads To Hell – Kenneth E Hagin

Measles Transmitted By The Vaccinated, Gov. Researchers Confirm

Cases of mumps hits 10-year high
COLORADO SPRINGS, Colo. (KXRM)— Top health officials are monitoring the worst mumps in the United States in 10 years.
Harvard University has been dealing with handfuls of infected students since the beginning of the school year and over at the University of Missouri, more than 200 people have come down with the virus.
The CDC says mumps cases have now surfaced in all but four states with nearly 4,300 infections reported.
Several public schools near Seattle have reported a growing number of cases.
Arkansas is the epicenter – with nearly 2,200 cases – in mostly school-aged children.
Mumps is extremely contagious and can be spread with a sneeze or cough.
Doctors say the best way to protect yourself is to get vaccinated.

Study – Difficulties in Eliminating Measles and Controlling Rubella and Mumps: A Cross-Sectional Study of a First Measles and Rubella Vaccination and a Second Measles, Mumps, and Rubella Vaccination
Background
The reported coverage of the measles–rubella (MR) or measles–mumps–rubella (MMR) vaccine is greater than 99.0% in Zhejiang province. However, the incidence of measles, mumps, and rubella remains high. In this study, we assessed MMR seropositivity and disease distribution by age on the basis of the current vaccination program, wherein the first dose of MR is administered at 8 months and the second dose of MMR is administered at 18–24 months

Public Health Officials Know: Recently Vaccinated Individuals Spread Disease
Washington, D.C., March 3, 2015 (GLOBE NEWSWIRE) — Physicians and public health officials know that recently vaccinated individuals can spread disease and that contact with the immunocompromised can be especially dangerous. For example, the Johns Hopkins Patient Guide warns the immunocompromised to “Avoid contact with children who are recently vaccinated,” and to “Tell friends and family who are sick, or have recently had a live vaccine (such as chicken pox, measles, rubella, intranasal influenza, polio or smallpox) not to visit.”1
A statement on the website of St. Jude’s Hospital warns parents not to allow people to visit children undergoing cancer treatment if they have received oral polio or smallpox vaccines within four weeks, have received the nasal flu vaccine within one week, or have rashes after receiving the chickenpox vaccine or MMR (measles, mumps, rubella) vaccine.2
“The public health community is blaming unvaccinated children for the outbreak of measles at Disneyland, but the illnesses could just as easily have occurred due to contact with a recently vaccinated individual,” says Sally Fallon Morell, president of the Weston A. Price Foundation. The Foundation promotes a healthy diet, non-toxic lifestyle and freedom of medical choice for parents and their children. “Evidence indicates that recently vaccinated individuals should be quarantined in order to protect the public.”
Scientific evidence demonstrates that individuals vaccinated with live virus vaccines such as MMR (measles, mumps and rubella), rotavirus, chicken pox, shingles and influenza can shed the virus for many weeks or months afterwards and infect the vaccinated and unvaccinated alike.

Measles Transmitted By The Vaccinated, Gov. Researchers Confirm
A remarkable study reveals that a vaccinated individual not only can become infected with measles, but can spread it to others who are also vaccinated against it – doubly disproving two doses of MMR vaccine is “99% effective,” as widely claimed.
One of the fundamental errors in thinking about measles vaccine effectiveness is that receipt of measles-mumps-rubella (MMR) vaccine equates to bona fide immunity against these pathogens. Indeed, it is commonly claimed that receiving two doses of the MMR vaccine is “99 percent effective in preventing measles,”1 despite a voluminous body of contradictory evidence from epidemiology and clinical experience.
This erroneous thinking has led the public, media and government alike to attribute the origin of measles outbreaks, such as the one recently reported at Disney, to the non-vaccinated, even though 18% of the measles cases occurred in those who had been vaccinated against it — hardly the vaccine’s claimed “99% effective.” The vaccine’s obvious fallibility is also indicated by the fact that that the CDC now requires two doses.
But the problems surrounding the failing MMR vaccine go much deeper. First, they carry profound health risks (over 25 of which we have indexed here: MMR vaccine dangers), including increased autism risk, which a senior CDC scientist confessed his agency covered up. Second, not only does the MMR vaccine fail to consistently confer immunity, but those who have been “immunized” with two doses of MMR vaccine can still transmit the infection to others — a phenomena no one is reporting on in the rush to blame the non- or minimally-vaccinated for the outbreak.
MMR Vaccinated Can Still Spread Measles
Last year, a groundbreaking study published in the journal Clinical Infectious Diseases, whose authorship includes scientists working for the Bureau of Immunization, New York City Department of Health and Mental Hygiene, and the National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, GA, looked at evidence from the 2011 New York measles outbreak that individuals with prior evidence of measles vaccination and vaccine immunity were both capable of being infected with measles and infecting others with it (secondary transmission).
This finding even aroused the attention of mainstream news reporting, such as this Sciencemag.org article from April 2014 titled “Measles Outbreak Traced to Fully Vaccinated Patient for First Time.”
Titled, “Outbreak of Measles Among Persons With Prior Evidence of Immunity, New York City, 2011,” the groundbreaking study acknowledged that, “Measles may occur in vaccinated individuals, but secondary transmission from such individuals has not been documented.”
In order to find out if measles vaccine compliant individuals are capable of being infected and transmitting the infection to others, they evaluated suspected cases and contacts exposed during a 2011 measles outbreak in NYC. They focused on one patient who had received two doses of measles-containing vaccine and found that,

Rise In Mumps Cases Has Some Public Health Officials Asking Questions
Caroline Brown, a sophomore at the University of Missouri got a fever over Thanksgiving break. Soon it became painful to bite down, and her cheek began to swell. A trip to her physician confirmed it – Caroline had the mumps.
“Mumps kind of sounds like this archaic thing,” Brown said. “We get vaccinated for it – it just sounds like something that nobody gets. So I just didn’t think that it was possible that I would get it.”
But mumps is back, and is having its worst year in a decade, fueled in part by its spread on college campuses. Since classes began at the University of Missouri in August, school officials have identified 193 mumps cases on campus, with more unreported cases likely. And nationwide more than 4,000 cases have been reported to the CDC, nearly triple the cases in 2015 and the largest spike in 10 years.
The spike has some public health officials asking questions about the existing vaccine protocol.
Dr. Susan Even, executive director for the University of Missouri’s Student Health Center said she hasn’t seen anything like the current outbreak in her 31 years at the school. She said all of the students her team treated for mumps had two MMR (Measles, Mumps and Rubella) vaccine doses — a school requirement — but they got sick anyway.
“The fact that we have mumps showing up in highly immunized populations likely reflects something about the effectiveness of the vaccine,” Even said.

“Official data have shown that the large-scale vaccinations undertaken in the US have failed to obtain any significant improvement of the diseases against which they were supposed to provide protection.” Dr A. Sabin, developer of the Oral Polio vaccine
Dr A. Sabin, developer of the Oral Polio vaccine (lecture to Italian doctors in Piacenza, Italy, December 7th 1985)

Study – Real-time RT-PCR assays to differentiate wild-type group A rotavirus strains from Rotarix® and RotaTeq® vaccine strains in stool samples
Abstract
Group A rotaviruses (RVA) are the leading cause of severe diarrhea in young children worldwide. Two live-attenuated RVA vaccines, Rotarix® and RotaTeq® are recommended by World Health Organization (WHO) for routine immunization of all infants. Rotarix® and RotaTeq® vaccines have substantially reduced RVA associated mortality but occasionally have been associated with acute gastroenteritis (AGE) cases identified in vaccinees and their contacts. High-throughput assays are needed to monitor the prevalence of vaccine strains in AGE cases and emergence of new vaccine-derived strains following RVA vaccine introduction. In this study, we have developed quantitative real-time RT-PCR (qRT-PCR) assays for detection of Rotarix® and RotaTeq® vaccine components in stool samples. Real-time RT-PCR assays were designed for vaccine specific targets in the genomes of Rotarix® (NSP2, VP4) and RotaTeq® (VP6, VP3-WC3, VP3-human) and validated on sequence confirmed stool samples containing vaccine strains, wild-type RVA strains, and RVA-negative stools. For quantification, standard curves were generated using dsRNA transcripts derived from RVA gene segments. Rotarix® NSP2 and VP4 qRT-PCR assays exhibited 92–100% sensitivity, 99–100% specificity, 94–105% efficiency, and a limit of detection of 2–3 copies per reaction. RotaTeq® VP6, VP3-WC3, and VP3-human qRT-PCR assays displayed 100% sensitivity, 94–100% specificity, 91–102% efficiency and limits of detection of 1 copy, 2 copies, and 140 copies, respectively. These assays permit rapid identification of Rotarix® and RotaTeq® vaccine components in stool samples from clinical and surveillance studies and will be helpful in determining the frequency of vaccine strain-associated AGE.

Health Department: Oklahoma mumps outbreak impacts vaccinated patients
Quick Facts:
State and county health departments investigating mumps outbreak
DOCUMENT: Dec 2016 Outbreak Info: http://mediaweb.fox23.com/document_dev/2016/12/15/Mumps%20Outbreak%20Web%20Update%20SEP19_6830847_ver1.0.pdf
Outbreak currently impacts areas of Garfield and Kay Counties
Cases in Canadian, McClain, Osage, Tulsa and Woods Counties connected to those outbreak areas
Vaccinated patients were also impacted by the outbreak
A recent outbreak of mumps in Oklahoma impacts even patients vaccinated against the disease.

Study: Real-time RT-PCR assays to differentiate wild-type group A rotavirus strains from Rotarix® and RotaTeq® vaccine strains in stool samples

Real-time RT-PCR assays to differentiate wild-type group A rotavirus strains from Rotarix® and RotaTeq® vaccine strains in stool samples
Abstract
Group A rotaviruses (RVA) are the leading cause of severe diarrhea in young children worldwide. Two live-attenuated RVA vaccines, Rotarix® and RotaTeq® are recommended by World Health Organization (WHO) for routine immunization of all infants. Rotarix® and RotaTeq® vaccines have substantially reduced RVA associated mortality but occasionally have been associated with acute gastroenteritis (AGE) cases identified in vaccinees and their contacts. High-throughput assays are needed to monitor the prevalence of vaccine strains in AGE cases and emergence of new vaccine-derived strains following RVA vaccine introduction. In this study, we have developed quantitative real-time RT-PCR (qRT-PCR) assays for detection of Rotarix® and RotaTeq® vaccine components in stool samples. Real-time RT-PCR assays were designed for vaccine specific targets in the genomes of Rotarix® (NSP2, VP4) and RotaTeq® (VP6, VP3-WC3, VP3-human) and validated on sequence confirmed stool samples containing vaccine strains, wild-type RVA strains, and RVA-negative stools. For quantification, standard curves were generated using dsRNA transcripts derived from RVA gene segments. Rotarix® NSP2 and VP4 qRT-PCR assays exhibited 92–100% sensitivity, 99–100% specificity, 94–105% efficiency, and a limit of detection of 2–3 copies per reaction. RotaTeq® VP6, VP3-WC3, and VP3-human qRT-PCR assays displayed 100% sensitivity, 94–100% specificity, 91–102% efficiency and limits of detection of 1 copy, 2 copies, and 140 copies, respectively. These assays permit rapid identification of Rotarix® and RotaTeq® vaccine components in stool samples from clinical and surveillance studies and will be helpful in determining the frequency of vaccine strain-associated AGE.

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Vaccine News – VAXXED TV – Something is Wrong & Study – Differentiation of RotaTeq® vaccine strains from wild-type strains using NSP3 gene in reverse transcription polymerase chain reaction assay